Pokello ea Mali ea Setšoantšo sa Gray Tube
Tlhaloso e Khutšoanyane:
Tube ena e na le potassium oxalate e le anticoagulant le sodium fluoride e le sebaballi - e sebelisetsoang ho boloka tsoekere maling le litekong tse ling tse khethehileng tsa k'hemistri.
Ha plasma e hlokahala, latela mehato ena.
1.Kamehla sebelisa "vacuum tube" e nepahetseng bakeng sa liteko tse hlokang anticoagulant e khethehileng (mohlala, EDTA,heparin,sodium citrate, joalo-joalo) kapa preservative.
2.Tlanya tube ka bonolo ho lokolla li-additive tse khomarelang tube kapa diaphragm ea stopper.
3.Lumella hore tube ea vacuum e tlale ka ho feletseng.Ho hlōleha ho tlatsa tube ho tla baka mali a sa lokelang-ho.karo-karolelano ea anticoagulant le ho fana ka liphetho tse belaetsang tsa liteko.
4.Ho qoba ho koala, kopanya mali le anticoagulant kapa preservative hang ka mor'a ho hula e 'ngoe le e' ngoeHo etsa bonnete ba ho kopanya ho lekaneng, butle-butle fetola tube ka makhetlo a mahlano ho isa ho a tšeletseng u sebelisa ho potoloha ha letsoho ha bonolo.tshisinyo.
5.Hang-hang centrifuge mohlala bakeng sa 5minutes. U se ke ua tlosa setopo.
6. Tima centrifuge 'me u e lumelle ho ema ka ho feletseng. U se ke ua e emisa ka letsoho kapa ka mariki.tube ka hloko ntle le ho senya bokahare.
7.Haeba ha u na tube e ka holimo e Botala bo Botala (Plasma Separator tube), tlosa setopo 'me u hahamalle ka hloko.plasma, o sebelisa pipette ea Pasteur e lahliloeng ka thoko bakeng sa tube ka 'ngoe. Beha ntlha ea pipette khahlanong le lehlakore.ea tube, hoo e ka bang 1/4 inch ka holim'a lera la sele. U se ke ua sitisa lesela la sele kapa ua tsamaisa liseleka har'a pipette.U se ke ua tšela; sebelisa pipette ea ho fetisa.
8.Fetisetsa plasma ho tloha pipette ho ea ho tube ea ho fetisa. Etsa bonnete ba hore u fana ka laboratori ka bongata baplasma e boletsoeng.
9. Ngola li-tubes tsohle ka ho hlaka le ka hloko ka lintlha tsohle tse amehang kapa khoutu ea bar. Li-tubes kaofela li lokela ho ngoloae nang le lebitso le felletseng la mokuli kapa nomoro ea boitsebahatso joalo ka ha e hlaha foromong ea kopo ea tlhahlobo kapa khoutu ea affix.Hape, hatisa leiboleng mofuta oa plasma e rometsoeng (mohlala, "Plasma, Sodium Citrate," "Plasma, EDTA," joalo-joalo).
10. Ha plasma e hatselitsoeng e hlokahala, beha li-tube tsa phetiso ea polasetiki hang-hang ka phaposing ea sehatsetsi easehatsetsing, 'me u tsebise moemeli oa hau oa litšebeletso hore u na le mohlala o hatselitsoeng o lokelang ho nkuoaholimo.
