Tube e le 'ngoe ea Lisele tsa Muclear Cell Separation Tube-CPT Tube

Tube e le 'ngoe ea Lisele tsa Muclear Separation Tube-CPT Tube Featured Image

Tlhaloso e Khutšoanyane:

E sebelisetsoa ho arola monocyte maling a felletseng.

E sebelisoa haholo-holo bakeng sa ho lemoha tšebetso ea 'mele ea ho itšireletsa mafung ea lymphocyte joalo ka HLA, ho fumana liphatsa tsa lefutso tsa leukemia le kalafo ea lisele tsa 'mele.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Seo e leng sona CPT Tube

Tube e le 'ngoe ea muclear cell seperation tube (CPT tube) e eketsoa ka hypaque, anticoagulant le gel ea karohano.Li-lymphocyte le lisele tsa mononuclear li ka aroloa habonolo ho mali a feletseng ka mohato o le mong oa centrifugation ha u sebelisa gel e khethehileng ea karohano ea lisele.E sebelisoa haholo bakeng sa ho lemoha tšebetso ea 'mele ea ho itšireletsa mafung ea lymphocyte, HLA kapa ho sibolla liphatsa tsa lefutso tsa leukemia le kalafo ea lisele tsa 'mele.E fana ka mokhoa o tloaelehileng oa mohato o le mong oa ho ntšoa ha monocyte bakeng sa ho lokisoa ha mohlala oa tlhahlobo ea bongaka le immunotherapy ea cellular.

Mosebetsi oa Sehlahisoa

1) Boholo: 13 * 100mm, 16 * 125mm;

2) Molumo oa Tlatsetso: 0.1ml, 135usp;

3) Bophahamo ba Mali: 4ml, 8ml;

4) Shelf Life: Likhoeli tse 24 ho tloha letsatsing la tlhahiso;

5) Polokelo:Boloka ho 18-25℃.

SehlahisoaMolemo

1) E sebetsa hantle, e nepahetse ebile e bolokehile;

2) Ka Ficoll Hypaque e hahiloeng, anticoagulant le gel ea karohano, lisele tsa mononuclear li arohanngoa le mali a feletseng ka mohato o le mong oa centrifugation.

3) Theknoloji e nepahetseng ea karohano ea lisele.

4) Theknoloji ea ho sebetsana le lebota le ka hare la bionic membrane;

5) Sekhahla sa ho hlaphoheloa ha monocyte se feta 90%, bohloeki bo feta 95%, 'me tekanyo ea ho pholoha e feta 99%.

Litaba Tse Lokelang ho Eloa Hloko

Lintlha tse latelang li lokela ho hlokomeloa:

1) Ha u etsa liteko tsa lisele tsa culturing, ela hloko ts'ebetso ea aseptic, sterilize li-reagents (tharollo ea karohano, tharollo ea ho hlatsoa, joalo-joalo) le lisebelisoa.Opereishene ena e lokela ho sebetsoa ke litsebi ho tiisa hore ts'ebetso e kopane.

2) Mocheso oa centrifugation hangata o le mocheso oa kamore (2 ~ 25 ℃).

3) Hangata ha ho arola lisele tsa mononuclear (PBMC) le Ficoll, palo ea sele e khubelu ea mali e nyenyane, e ka etsang hore tlhahlobo e be e nepahetseng.Kahoo lysate e ka sebelisoa (tse ling li hloka ho etsoa sterilized), laola nako ea lysis le ho thibela ho ama ts'ebetso ea lisele tsa mononuclear.

4) E-ba hlokolosi mabapi le "centrifugation" hape e ka khonang habeli ho hlapolla.

E fetileng: CTAD Detection Tube

E 'ngoe: Setsi sa Mali se Sefubelu sa Plain