Platelet-rich plasma (PRP) ke motsoako o ikemetseng oa liplatelete tsa motho ka har'a plasma.Ka ho senyeha ha li-alpha granules ka liplatelete, PRP e ka boloka lintlha tse sa tšoaneng tsa kgolo, ho kenyelletsa le platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), le ho fetola growth factor (TGF), e ngotsoeng ho qalisa pholiso ea maqeba le ho khothalletsa ho ata le ho fetoha ha lisele tsa endothelial le li-pericyte hore e be limela tsa endothelial.
Mesebetsi ea PRP bakeng sa phekolo ea ho hōla ha moriri e tlalehiloe liphuputsong tse ngata tsa morao tjena.Uebel et al.ba fumane hore lintlha tsa khōlo ea platelet plasma li eketsa chai ea likarolo tsa follicular ho opereishene ea lefatla ea banna.Mosebetsi oa morao-rao o bontšitse hore PRP e eketsa ho ata ha lisele tsa dermal papilla le ho etsa hore phetoho e potlakileng ea telogen-to-anagen e sebelise mefuta ea vivo le in vitro.Phuputso e 'ngoe e bontšitse hore PRP e khothalletsa ho tsosolosoa ha moriri oa moriri le ho khutsufatsa haholo nako ea ho thehoa ha moriri.
Ka bobeli PRP le plasma e futsanehileng ea platelet (PPP) e kenyelletsa tlatsetso e felletseng ea liprotheine tsa coagulation.Thutong ea hona joale, tšusumetso ea PRP le PPP ka kholo ea moriri ho C57BL / 6 litoeba e ile ea batlisisoa.Khopolo-taba e ne e le hore PRP e na le phello e ntle ho kholo ea bolelele ba moriri le ho eketseha ha palo ea moriri oa moriri.
Liphoofolo tsa liteko
Ka ho feletseng litoeba tse 50 tse phetseng hantle tsa C57BL/6 (libeke tse 6, 20 ± 2 g) li ile tsa fumanoa Setsing sa Liphoofolo tsa Laboratori, Hangzhou Normal University (Hangzhou, Chaena).Liphoofolo li ne li feptjoa lijo tse tšoanang ’me li bolokoa sebakeng se sa khaotseng tlas’a potoloho ea khanya le lefifi ea lihora tse 12:12.Ka mor'a beke ea 1 ea acclimatization, litoeba li ne li arotsoe ka lihlopha tse tharo: sehlopha sa PRP (n = 10), sehlopha sa PPP (n = 10), le sehlopha sa taolo (n = 10).
Protocol ea boithuto e amohetsoe ke komiti ea melao ea boitšoaro ea setheo ea lipatlisiso tsa liphoofolo tlasa Molao oa Lipatlisiso tsa Liphoofolo le Melao ea Molao naheng ea China.
Tekanyo ea bolelele ba moriri
Matsatsing a 8, 13, le a 18 ka mor'a ente ea ho qetela, moriri oa 10 ho mouse e 'ngoe le e' ngoe e khethiloe ka mokhoa o sa reroang sebakeng seo ho shebiloeng ho sona.Litekanyo tsa bolelele ba moriri li ne li etsoa masimong a mararo ho sebelisoa microscope ea elektronike, 'me karolelano ea tsona e ne e hlahisoa e le limilimithara.Moriri o molelele kapa o senyehileng o ne o sa kenyelletsoa.
Hematoxylin le eosin (HE) staining
Mehlala ea letlalo la dorsal e ile ea hlajoa matsatsing a 18 ka mor'a ente ea boraro.Joale lisampole li ile tsa lokisoa ka 10% e sa nke lehlakore e buffered formalin, e kentsoeng ka parafineng, 'me ea khaoloa ka 4 μm.Likarolo li ile tsa behoa lihora tse 4 bakeng sa deparaffinization ka 65 ° C, li kenngoa ka har'a ethanol ea gradient, ebe li silafatsoa ka hematoxylin bakeng sa 5 min.Ka mor'a ho aroloa ka 1% ea joala ea hydrochloric acid, likarolo li ne li kenngoa metsing a ammonia, a silafalitsoeng ka eosin, 'me a hlatsuoa ka metsi a silafetseng.Qetellong, likarolo li ile tsa felloa ke metsi ka ethanol ea gradient, e hloekisitsoeng ka xylene, e kenngoa ka resin e sa nke lehlakore, 'me ea hlokomeloa ho sebelisa microscopy e khanyang (Olympus, Tokyo, Japan).
Nako ea poso: Oct-12-2022