PRP Tubes Acd Tubes
Tlhaloso e Khutšoanyane:
Anticoagulant Citrate Dextrose Tharollo, eo ka tloaelo e tsejoang e le ACD-A kapa Tharollo A ke tharollo e seng pyrogenic, e nyopa.Karolo ena e sebelisoa e le anticoagulant ha ho etsoa plasma e nang le platelet-rich (PRP) e nang le PRP Systems bakeng sa ts'ebetso ea mali ea extracorporeal.
Anticoagulant Citrate Dextrose Tharollo, eo ka tloaelo e tsejoang e le ACD-A kapa Tharollo A ke tharollo e seng pyrogenic, e nyopa.Karolo ena e sebelisoa e le anticoagulant ha ho etsoa plasma e nang le platelet-rich (PRP) e nang le PRP Systems bakeng sa ts'ebetso ea mali ea extracorporeal.Li-anticoagulants tse thehiloeng ho citrate li sebelisa bokhoni ba citrate ion ho chelate ionized calcium e teng maling ho thibela ho kopana ha mali le ho theha motsoako o se nang ionized calcium-citrate.
Sehlahisoa se le seng feela sa anticoagulant seo Tsamaiso ea Lijo le Lithethefatsi ea United States e lumeletseng hore e sebelisoe bakeng sa ho lokisoa ha PRP ka mekhoa e fapaneng ea PRP Systems ke ACD-A.Ho latela lipatlisiso tse entsoeng ka 2016 ho PRP e fumanoeng ka li-anticoagulant tse fapaneng le litlamorao tsa tsona boitšoarong ba lisele tsa mesenchymal stromal in vitro le linomoro tsa platelet, ho na le litholoana tse ntle ts'ebelisong ea PRP bakeng sa tokiso ea mesifa ea mesifa.
Bakeng sa ho arola liplatelete ho eletsoa ho nkela sebaka sa sodium citrate e tloaelehileng bakeng sa Acid Citrate Dextrose (ACD-A) kaha mokhoa oa ho itšehla thajana o hloka mehato e mengata ea ho hlatsoa.Li-platelet li tsitsitse ho 37C nakong ea ho ohla, empa ho li ohla mochesong oa kamore (25 C) le hona ho sebetsa hantle.Ho fokotsa pH ka ACD-A (e atamela haufi le 6.5) ho thusa ho sitisa ts'ebetso ea mesaletsa ea thrombin ka har'a li-platelet tubes, 'me ho tlatsetsa tlhokomelong e akaretsang ea morphology ea platelet ha o ntse o fetola mosebetsi ho minima.Ka tloaelo o hloka ho khutlisetsa liplatelete ho Tyrode Buffer e nepahetseng (pH 7.4) e le hore u boele u sebetse.ACD e na le melemo e mengata ha ho tluoa tabeng ea ho boloka liplatelete
Ha ACD e sebelisoa, liphetho li bonts'a chai e phahameng ea platelet maling ka kakaretso.Leha ho le joalo, ts'ebeliso ea EDTA e boetse e khothalelitse kholo ea palo e bolelang ea platelet ka mor'a hore mehato ea mali ea centrifugation e phethoe ho fumana PRP.Ka mor'a moo, tšebeliso ea ACD e ile ea fella ka ho ata ha lisele tsa mesenchymal stromal.Ka hona, ho ile ha fihleloa qeto ea hore li-anticoagulants tse kenyeletsang ACD-A li phetha karolo e kholo ho lokiseng PRP le ho thusa ho ntlafatsa ts'ebetso ka tekanyo e kholo.
